Mechanism of IgA-albumin complex formation that affects the fructosamine assay

Fructosamine assay using albumin extracted from serum.

Albumin extracted from serum by a simple technique using trichloroacetic acid and ethanol has been applied to a fructosamine assay using nitroblue tetrazolium. A fructosamine assay using extracted albumin sample was carried out without interference from low molecular weight substances with reducing activities and other proteins with varying concentrations, half-lives and reducing activities. 1-...

Effects of albumin and immunoglobulin A on fructosamine assay.

Serum fructosamine, albumin, and IgA were measured in three groups of human subjects: 54 nondiabetic normal individuals, 149 nondiabetic patients, and 149 diabetic patients. Normal subjects had significantly (P less than 0.05) higher fructosamine (2.91, SD 0.33, mmol/L) than did nondiabetic patients (2.49, SD 0.46, mmol/L). Each of these groups had significantly (P less than 0.05) lower fructos...

Influence of timing in the fructosamine assay.

The fructosamine assay, based on the measurement of the reducing activity in serum at alkaline pH, provides an index of protein glycation. The reducing activity is expressed in equivalents of 1-deoxy-1-morpholinofructose (DMF) by direct comparison with the activity either of this synthetic compound or with a secondary protein standard calibrated against DMF. This study reports the influence of ...

formation and evolution of regional organizations: the case study of the economic cooperation organization (eco)

abstract because of the many geopolitical, geo economical and geo strategically potentials and communicational capabilities of eco region, members can expand the convergence and the integration in base of this organization that have important impact on members development and expanding peace in international and regional level. based on quality analyzing of library findings and experts interv...

Immune complex formation in IgA nephropathy: